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cell lines 231mfp  (ATCC)
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ATCC cell lines 231mfp
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Cell Lines 231mfp, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Journal: Cell chemical biology

Article Title: Covalent Ligand Discovery Against Druggable Hotspots Targeted by Anti-Cancer Natural Products

doi: 10.1016/j.chembiol.2017.08.013

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Article Snippet: Data and Software Availability N/A Additional Resources N/A REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Vinculin Abcam ab129002 Rabbit anti-p-Akt (S473) CST D9E Mouse anti-total Akt CST 40D4 Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins Withaferin A Sigma W4394 N-Hex-5-ynyl-2-iodo-acetamide (IAyne) Chess Gmbh 3187 Sequencing grade trypsin (porcine) Promega V5111 AcTEV protease Invitrogen 12575015 1,4-benzodioxan- 6-amine Sigma 193232 Chloroacetyl chloride Sigma 104493 Triethylamine Sigma T0886 Sodium Hydride Sigma 452912 Benzyl Bromide Sigma {"type":"entrez-nucleotide","attrs":{"text":"B17905","term_id":"2125654","term_text":"B17905"}} B17905 5,6,7,8-Tetrahydro-2-napthylamine Sigma 678864 Biotin-TEV-azide ( Weerapana et al., 2010 ) Critical Commercial Assays Malachite Green Phosphate Assay Kit Cayman 10009325 Hoechst 33342 dye Invitrogen H3570 Deposited Data Experimental Models: Cell Lines 231MFP ( Jessani et al., 2004 ) HCC38 ATCC CRL-2314 MCF7 ATCC HTB-22 MCF10A ATCC CRL-10317 HEK293T/17 ATCC CRL-11268 Experimental Models: Organisms/Strains C.B17 SCID mice Taconic CB17SC-F Oligonucleotides PPP2R1A SMARTPOOL siRNA Dharmacon L-010259-00-0005 Recombinant DNA PPP2R1A (Myc-DDK-tagged) expression plasmid Origene RC200056 PPP2R2A (Myc-DDK-tagged) expression plasmid Origene MR207137 Software and Algorithms IP2 proteomics pipeline 4.1.1 Integrated Proteomics Applications Other Streptavidin agarose ThermoFisher 20347 Open in a separate window Key Resources Table

Techniques: Virus, Recombinant, Sequencing, Expressing, Plasmid Preparation, Software